sanne,
To add to godthe's reply.
Under the sand should be 150mm of stone.
In your concrete screed you will have your plumbing pipes if you will have rad.s in your extension.
I would also ensure that you run 25mm vertical insulation around the perimeter walls the depth of your concrete screed - avoiding cold bridging.
You may also need to install one of those radon boxes in the stone with a 4'' pipe coming out of your external wall.I don't believe in them myself but just in case you ever wanted to sell it sounds good.