Your queries are very hard to decipher.
I think this is what you're saying. I'm married and separating. I own two properties in my sole name. I hope to sell them both to a third party. Or I intend to transfer the family home to my wife and she will pay me a lump sum. I will then sell or keep the investment property.
If you sell everything to a third party, you will have estate agents and legal fees on the sales. You may have capital gains tax on the sale of the investment property. You can give your spouse any amount of money tax free.
If you transfer the family home to your spouse, there are no tax issues. If there is a mortgage, the bank may not permit the transfer. You will have legal fees.
If either of you buy another property, you will have stamp duty to pay and legal fees.
You really, really, really need to be talking to your own solicitor.
mf