If I understand your somewhat convoluted description correctly, you have a single through ticket DUB-LGW-MCO. With a single ticket covering the entire journey, if you do not show up for any leg, the remainder of the ticket will automatically be canceled. So, yes, as you suspected - if you do not use the Gatwick flight, you will lose your Orlando flight as well.
If the transfer time is short, your Gatwick flight is delayed and you miss your onward connection, you will lose time but no money. Since you have a through ticket, it is not your problem to book a different flight. The airline will do it for you.